美國Hausser細胞計數板

美國Hausser細胞計數板

美國Hausser細胞計數板（Nageotte Counting Chamber）：亮線型，常用于單采血小板中的殘留白細胞的計數。池深：0.5mm。提供兩種：一種H型，中間有溝隔開為兩計數區（貨號4000），一種沒有溝槽（4001）美國Hausser scientific血球計數板，霍華德霉菌計數板，準確計算白細胞、紅細胞、血小板、組織培養細胞、細菌和精子The Hausser Nageotte Bright-Line Hemacytometer allows one to manually count low leukocyte concentrations below the capabilities of a standard Hemacytometer. The Nageotte contains two gridded counting areas each containing 40 rectangular areas. Total volume of each grid is 50 mL for a total volume of 100 mL per Nageotte. The Nageotte can accuray measure down to 0.1 cell/mL (100 cell/ml). Cell Depth is 0.5mm .

美國Hausser細胞計數板

Available with an H-shaped moat(catalog#4000) that separates the two gridded areas or without a moat (catalog#4001). The Nageotte Chamber comes with two reusable cover slips(30mm X 33mm X 0.5mm) and 1/2 oz (approx. 35 pcs.) of disposable cover slips (30mm X 33mm X 0.15mm). PROCESS: Counting low levels of white cells in white cell-reduced plaetcomponents.BACKGROUND: Determination of the white cell (WBC) count in WBC-reduced plaet components requires methods that have a detection limit in the range of approximay 5.0 x 10(2) to 5.0 x 10(4) per mL.STUDY DESIGN AND METHODS: With a 50-microL Nageotte hemocytometer and bright-field microscopy (200x magnification), studies were conducted to develop and validate a method that could be used routinely with filtered and apheresis-harvested plaets. A 1-in-5 dilution of sample with a commercially available blood-diluting fluid was used because, with a lower (1-in-2) dilution, the observed number of WBCs was substantially less than the number expected at relatively high plaet counts (> 1.9 x 10(9)/mL). RESULTS: The observed and expected WBC counts in WBC-reduced plaet samples correlated well at levels between approximay 5 and 1100 WBCs per counting area (5.0 x 10(2)-1.1 x 10(5)/mL). At levels of more than 300 to 400 WBCs per counting area, accurate counts were obtained when 10 of the 40 rectangles were counted.